VNO Atlas Figure 1

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This file contains statistics and figures from the paper, “Molecular, Cellular, and Developmental Organization of the Mouse Vomeronasal organ at Single Cell Resolution” by Max Hills Jr., Limei Ma, Ai Fang, Thelma Chiremba, Seth Malloy, Allison Scott, Anoja Perera, and C. Ron Yu.

The paper can be found here:

Molecular, Cellular, and Developmental Organization of the Mouse Vomeronasal organ at Single Cell Resolution

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Single cell transcriptomic profile of the whole vomeronasal organ

What cell types, in what quantities, do we find in the whole VNO?

[1] "There are 34519 cells in the whole VNO dataset."

cell type	nCells
V1R_mVSN	13201
V2R_mVSN	4366
SUS	3889
sVSN	2601
V1R_iVSN	1794
V2R_iVSN	1582
Microglia	1366
Immune_Fpr	1222
LP	1072
HBC	912
INP	528
Microglia_rp	455
mOSN	430
T_cells	313
Endothelial	251
OEC	220
GBC	218
MV	99

2D PCA of Clusters

2D PCA of Cell-Types

3D PCA of Clusters

3D PCA of Cell-Types

2D UMAP Clusters

2D UMAP visualization of integrated Cell-Type clusters for whole-VNO single-cell RNA-seq

In two-dimensional UMAP space, 18 cell clusters can be clearly identified.

3D UMAP Clusters

3D UMAP Cell-types

Cell-type marker-gene normalized expression across the cell clusters

These clusters were curated using known cell markers (Fig. 1b).

UMAP of cell-type clusters split by age

UMAP of cell-type clusters split by sex

A representative image of transcript distribution from Molecular Cartography© platform, Resolve Biosciences

Spatial location of individual VNO cells color-coded according to cell type prediction based on the spatial transcriptomic analysis

Location of cell belonging to HBC, GBC, INP, and LP cell types, respectively. Heat indicates confidence of predicted values